Composite

Part:BBa_K2539400:Design

Designed by: Justin Wu   Group: iGEM18_TAS_Taipei   (2018-09-03)


PalcA-Regulated ALDH2*1 Expression Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 274
    Illegal BglII site found at 2116
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1174
    Illegal NgoMIV site found at 1317
    Illegal NgoMIV site found at 1830
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1236
    Illegal SapI.rc site found at 1733


Design Notes

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Source

This construct was created to regulate ALDH2*1 production by using PalcA. We acquired all parts from the iGEM distribution kit: an inducible promoter and strong RBS (BBa_K2092002 and BBa_B0034) to regulate ALDH2*1 production, ALDH2*1 (BBa_M36520) and a double terminator (BBa_B0015) to end transcription.


References